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安科爾文翻譯中翻譯英課程名稱︰分子生物學 課程性質︰必修 課程教師︰主授張麗冠（此範圍為張麗冠/林甫容老師授課） 開課學院：生命科學院 開課系所︰生化科技學系 考試日期（年月日）︰2017/01/09 考試時限（分鐘）：100分鐘 試題 : (以下敘述均非與原題目完全相同，僅就考試主題作最接近 翻譯題目描述) 考試範圍：Ch 18~21 + Ch 15 (regulation部分) Transcriptional Regulation in Prokaryotes Transcriptional Regulation in Eukaryotes Control of Translation Regulatory RNAs 。-> 翻譯社|,-> 翻譯公司|的-> 翻譯Gene Regulation in Development and Evolution 張老師部分：佔79% (Ch 18~20 + Ch 15 regulation部分) 1. Define the following terms. (30%) (1) Dicer (2) Heterochromatin Protein 1 (3) Aptamer (4) Nonsense-mediated decay (5) Internal Ribosome Entry Site (6) Insulator (7) Super Elongation Complex (SEC) (8) Ribozyme switches (9) RNase P (10) Two component system and phosphorelay 2. Please describe the whole process of miRNA formation 翻譯公司 as well as how it is used to function in transcriptional gene silencing. Please also specify the primary components it will use throughout formation. (8%) 3. How do you distinguish regulation in transcriptional initiation and translational initiation? (8%) ※ Tip: 可用實驗設計說明 4. How are eIF2 and eIF4E involved in regulation of transcriptional initiation in eukaryotes? (6%) 5. What is type II CRISPR-Cas immunity in bacteria? (8%) (附上第七版課本兩張圖： 翻譯社-> 翻譯社| 翻譯公司-> 翻譯公司| 翻譯-> 翻譯 Figure 18-21: Map of bacteriophage λ in the circular form. Figure 18-30: Genes and promoters involved in the lytic/lysogenic choice.) 6. How does a phage regulate itself to enter lysogenic phase?(6%) How does it regulate the expression of integrase (Int) and excisionase (Xis)? (6%) 7. Read the following article and answer the questions. The histone chaperone Spt6 coordinates histone H3K27 demethylation and myogenesis. EMBO J. 2013 Apr 17;32(8):1075-86. Abstract Histone chaperones affect chromatin structure and gene expression through interaction with histones and RNA polymerase II (PolII). Here 翻譯公司 we report that the histone chaperone Spt6 counteracts H3K27me3, an epigenetic mark deposited by the Polycomb Repressive Complex 2 (PRC2) and associated with transcriptional repression. By regulating proper engagement and function of the H3K27 demethylase KDM6A (UTX), Spt6 effectively promotes H3K27 demethylation 翻譯公司 muscle gene expression, and cell differentiation. ChIP-Seq experiments reveal an extensive genome-wide overlap of Spt6 翻譯公司 PolII, and KDM6A at transcribed regions that are devoid of H3K27me3. Mammalian cells and zebrafish embryos with reduced Spt6 display increased H3K27me3 and diminished expression of the master regulator MyoD 翻譯公司 resulting in myogenic differentiation defects. As a confirmation for an antagonistic relationship between Spt6 and H3K27me3 翻譯公司 inhibition of PRC2 permits MyoD re-expression in myogenic cells with reduced Spt6. Our data indicate that, through cooperation with PolII and KDM6A, Spt6 orchestrates removal of H3K27me3 翻譯公司 thus controlling developmental gene expression and cell differentiation. (1) Define the function of CRP2. (3%) (2) What is the function of H3K27me3? (3%) (3) What is the function of Spt6? (3%) ------------- 林老師部分：佔25% (Ch 21) 1. Please explain the restricted expression of rhomboid in the ventral neurogenic ectoderm. (5%) 2. Given the homozygous mutants below, please use "normal", "dorsalized" or "ventralized" to describe what results you expect to see. (1) Homozygous toll mutant (2%) (2) Homozygous cactus mutant (2%) 3. What is the mechanism of localization of bicoid mRNA during oocyte? (You can draw if drawing can help you) (5%) 4. Please describe the feature of Hunchback protein gradient due to the distribution of Bicoid and Nanos that can explain for the proper embryo formation. (8%) 5. You are screening the mRNA expression in the pair-rule gene even-skipped (eve) with a homozygous mutant in Ubx gene that is loss of function. Please describe the result you may expect to see. (3%)

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